We genotype samples regardless whether we designed and validated the reagents for the modifications. And mouse samples are not limited to founders. In short, we will genotype your samples if you tell us what to look for.

Sample types: genomic DNA preps, cell pellets, animal tissues

Modification types:

KO, deletion, point mutations/tagging are genotyped by using NGS on PCR amplicons at the target locus

Large insertions are detected by using junction PCRs. See the example below to insert Cre-ETR2 at the N-ter of Pmp2 protein by introducing a cut at the ATG codon and a donor with insertion flanked by homology arms (HA-L and HA-R)

Donor

Final genomic locus is amplified with two sets of primers for 5’ and 3’ junctions, respectively, to demonstration site-specific insertion

STR profiling for cell line authentication

Many cell lines look similar, and cell lines are often mis-labelled or mistaken for one another. Errors about the identity and authenticity of cell lines can propagate and go unnoticed for years, fouling publications, complicating the integrity and reproducibility of results, and disrupting scientific enterprise. STR Profiling (Short Terminal Repeat Profiling) is a cell fingerprinting method originally applied to DNA forensic analysis, which profiles the number of repeats across multiple highly variable regions across the genome. STR profiles are unique to each cell line’s origin, ancestry, and genetic history, and is invaluable for authenticating the exact identity of your cell line.

NGS Submission Site

*if you need assistance with the NGS Submission Site, please reference these instructions