Ready-to-go Addgene libraries
NGS library prep and submission to GTAC, bioinformatics support Available as maxipreps of plasmid libraries, representation confirmed by NGS and ready for viral production.
Addgene libraries available to WU users
Custom library design and construction
gRNA designs for custom libraries are cherry-picked from published whole-genome libraries and cloned into the backbone of your interest. The resulting plasmid libraries are maxiprepped and sequenced to confirm representation, ready for viral production.
Post screen analysis
We prepare NGS libraries from your post-screen samples and submit to GTAC@MGI on your behalf.
Analyze sequencing data
To submit CRISPR reagent requests, go here, if you are on WashU network. Instructions can be found here. If you are a WU employee working off site or a non-WU user, you have to connect to the WashU network via VPN. For non-WU users, if this is the first time to use the site, you have to request a guest WUSTL key for VPN first by clicking here. Please use your institutional email address for the request. And if you are not the PI, please specify both your institution and lab under “Organization”. Sponsor’s email address: x.cui@wustl.edu. You will receive your credentials via email once the request is approved. And the credentials will only allow you to access this specific server. Please use Contact us to report any issues.