Ready-to-go Addgene libraries

NGS library prep and submission to GTAC, bioinformatics support Available as maxipreps of plasmid libraries, representation confirmed by NGS and ready for viral production. 

Addgene libraries available to WU users

Custom library design and construction

gRNA designs for custom libraries are cherry-picked from published whole-genome libraries and cloned into the backbone of your interest. The resulting plasmid libraries are maxiprepped and sequenced to confirm representation, ready for viral production.

Post screen analysis

We prepare NGS libraries from your post-screen samples and submit to GTAC@MGI on your behalf.

Analyze sequencing data

Submit CRISPR Library Requests

To submit CRISPR reagent requests, go here, if you are on WashU network. Instructions can be found here. If you are a WU employee working off site, you have to connect to the WashU network via VPN. For non-WU users, please contact us.

Link: The Hope Center Viral Vectors Core